Gfp Lc3 Cell Line

Also this cell line provides a useful tool for the study of autophagy using gfp lc3 as a standard assay read out by both immunofluorescence and biochemical methods.

Gfp lc3 cell line.

So far many gfp lc3 expressed cell line from different species have been used to measure their autophagy activities including humans other mammals birds and fishes. Single cell clones were selected and grown up. The raw264 7 cells stably expressing gfp lc3 white were incubated in complete media control and in earle s balanced salts solution starve or simultaneously supplemented with wortmannin starve wort for 1 h. Also this cell line provides a useful tool for the study of autophagy using gfp lc3 as a standard assay read out by both immunofluorescence and biochemical methods.

The canine gfp lc3 cell line established in this study can be not only used to detect the autophagic activity of dogs but also used to detect other canine animals such as. However i can not get a very high transfection efficiency 10 and this expression disappears after 48hrs. Raw264 7 cells were transfected with pegfp lc3 plasmids. Single cell clones were selected and grown up.

These cells derived from the murine raw 264 7 macrophage cell line express the rfp gfp lc3 fusion protein where the murine lc3b microtubule associated protein 1 light chain 3 beta is fused to two fluorescent reporter proteins. Production details egfp lc3 rat plasmid was transfected into hek293a cells. The lc3b protein is a good marker to assess the autophagic flux since. Single cell clones were selected and grown up.

This cell line uses gfp lc3 as a standard assay read out and can be evaluated by both immunofluorescence and biochemical methods. To generate the cell line egfp lc3 rat plasmid was transfected into hek293a cells. I am trying to transfect thp 1 cells with gfp lc3 to create a stably expressing cell line. To generate the cell line egfp lc3 rat plasmid was transfected into hek293a cells.

The cells were selected with 800ug ml geneticin. The cells were selected with 800ug ml geneticin.